Part:BBa_K4165208
Coh2 linked with tau binding peptide (M4W39) and GSGS linker
Fusion protein consists of Tau binding peptide (M4W39) for tau targeting and Coh2 protein [BBa_K4165003] that bind to Docs [BBa_K3396000] to form the protac system with linker GSGS [BBa_K4165065]
Usage and Biology
Protac system is known to be a promising one for targeting and signalling many diseases and biological application so according to our project aim. we introduced this fusion protein with different linker length and tau binding peptide to be a target for degradation of Tau aggregations. Furthermore, that can be validated by wet-lab results by in vitro or in vivo testing.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
3D Modeling and Ranking
This fused protein was modeled using Alphafold2.0 software and after running our quality assessment code it got a score of 4 out of 6 according to the parameters of the QA code: C-beta deviations, clash score, Molprobity, Ramachandran favored, Ramachandran outliers, Q mean 4, and Q mean 6. This protein was excluded due to its low QA score as compared to other models.
Figure 1. Predicted 3D structure of CoH2-(GSGS)-M4W39 protein Visualized by Pymol.
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